Barca saw their record-breaking unbeaten run come to an end after going down 2-1 to 10-man Real at the Nou Camp, with Cristiano Ronaldo netting an 85th-minute winner.
It was a chastening defeat against their biggest rivals but, with a six-point lead in the Primera Division, a Copa del Rey final to look forward to and a crunch Champions League quarterfinal against Atletico to play, defender Pique insists there is no need to blow the result out of proportion.
The Spain international, who headed Barca into a 56th-minute lead against Real, told a press conference on Monday: "We don't need a wake-up call because we already know how difficult and complicated it is to win every game.
However, Atletico will have fond memories of their last European clash with Barca, having knocked their Spanish rivals out at the same stage of the competition two years ago.
That is the only time in the last eight years that Barca have failed to reach the semi-finals, and Pique knows just how tough a nut Diego Simeone's side are to crack.
He said: "We're not scared, but they are having a great season. It's going to be more difficult than Saturday.
"They are the second best team in Spain, according to the league standings. But in the end it depends on us, that we have the ball, the support of the fans... If everything comes together, we're unstoppable."
Barca coach Luis Enrique echoed those thoughts, saying: "Atletico Madrid are a top-level team, that's why they are where they are competing with Barca and Madrid despite not having the financial resources.
"They are going to make life difficult for us regardless of Saturday's result."
Holders Barca will be without injured defenders Jeremy Mathieu, Aleix Vidal and Adriano for the match, which could see Lionel Messi reach 500 career goals. The Argentina ace is currently on 499 for club and country.
Atletico will be determined to ensure that does not happen on Tuesday night and they have been boosted by the return to fitness of key centre-back Diego Godin.
Godin has missed the last two games with a hamstring injury but was given the all-clear to return on Monday.
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